Assembly of a DNA Origami Chinese Knot by Only 15% of the Staple Strands

As a giant leap in DNA self-assembly, DNA origami has exhibited an unprecedented ability to construct nanostructures with arbitrary shapes and sizes. In typical DNA origami, hundreds of short DNA staple strands fold a long, single-stranded (ss) DNA scaffold cooperatively into designed nanostructures. However, large numbers of DNA strands are expensive and would hinder applications such as pharmaceutical investigations because of the complicated components. Therefore, one challenge is how to reduce the number of staple strands needed to construct DNA origami. For a DNA origami structure, the scale-free folding pattern of the scaffold strand is determined by staple strands at the branching vertexes. Simple duplex regions help to define the size-related features of the origami geometry. In this study, we hypothesized that a scaffold strand can be correctly folded into a designed topology by using only staple strands involved in branching vertexes. After assembly, any remaining, flexible, single-stranded regions of the scaffold could be converted into rigid duplexes by DNA polymerase to achieve the designed geometric structures. To demonstrate the concept, we used only 18 staple strands (covering 15 % of the scaffold strand) to assemble a porous DNA nanostructure, which was visualized by atomic force microscopy (AFM). This study helps understanding of the role of cooperativity in origami folding, and provides a cost-effective approach for small-scale prototyping DNA origami.     

Theoretical study of the structural,elastic and thermodynamic properties of chalcopyrite ZnGeP2

The structural, elastic, and thermodynamic properties of ZnGeP₂ with chalcopyrite structure are investigated using the pseudo-potentials plane wave method based on the density functional theory with the generalized gradient approximation. The lattice parameters (a, c and u) are directly calculated and agree well with previous experimental and theoretical results. The obtained negative formation enthalpy shows that ZnGeP₂ crystal has strong structural stability. We have also calculated the bulk modulus B and the elastic parameters (C₁₁, C₁₂, C₁₃, C₃₃, C₄₄, and C₆₆) which have not been measured yet. The accuracy and reliability of the calculated elastic constants of ZnGeP₂ crystal are discussed. In addition, the pressure and temperature dependencies of the lattice parameters, bulk modulus, Debye temperature, Grüneisen parameter, entropy, volume thermal expansion coefficient, and specific heat capacity are obtained in the ranges of 0–20 GPa and 0–1200 K using the quasi-harmonic Debye model. To our knowledge this is the first quantitative theoretical prediction of the thermodynamic properties for ZnGeP₂ compound and still awaits experimental confirmations.     

A commodity market algorithm for pricing substitutable Grid resources

A crucial goal for future Grid systems is to strive towards user-centric service provisioning. A way to achieve this is through the use of economics-based resource management. Currently, several models exist from among which auction- and commodity-based models are the most popular. This contribution will focus on the latter, and in particular on commodity markets, where the value of a Grid resource is determined by supply and demand. We propose some refinements to the application of Smale’s method for finding price equilibria in such a Grid market. We also extend the approach to substitutable goods. That is, we introduce ‘slow’ and ‘fast’ CPUs, two categories of the same type of good that are priced separately, but are strongly coupled with potentially strong shifts in demand. We show that Smale’s method can be adapted to handle this type of Grid resources market, and that price stability, allocative efficiency, and fairness are realized.     

结合模型匹配与特征跟踪的人体上半身三维运动姿态恢复方法

人体姿态空间的高维性及单目视频深度信息丢失,导致从单目视频恢复人体三维运动姿态非常困难,为此,利用特征跟踪的快速性及模型匹配的鲁棒性,提出一种无标记人体上半身三维运动跟踪方法.该方法利用匹配SIFT特征,并根据长度不变性约束建立优化目标函数,再采用迭代优化算法得到全局运动位姿;其他关节的姿态先根据逆运动学计算初始估计值,并通过模型匹配验证其可信度,当初始姿态估计错误时,则使用局部搜索获得关节姿态.实验结果表明,文中方法可以准确地恢复单目视频中人体上半身三维运动姿态.     

Analysis on the spatial ecological distribution model of landscape plant community

Because the spatial pattern of plant communities in garden landscapes usually exhibits highly non-random distribution characteristics, the accuracy of the analysis results is not high. For this purpose, a spatial ecological distribution model of landscape landform plant communities is designed. Based on the remote sensing images of landscape plant communities, the study area was obtained. The grassland communities in this area were classified and calculated by geostatistics, and the semi variogram values of typical samples of different vegetation types were obtained. According to the calculation results, the spatial terrain factors of landscape plant community are extracted, and the annual NDVI value is taken as the ecological vitality index of landscape green vegetation, the NDVI level is divided, the spatial ecological distribution model of landscape plant community is constructed, and the evaluation index system is generated, so as to complete the spatial ecological distribution model analysis of landscape plant community. The results show that the method of this study accurately analyzes the area of coniferous and broad-leaved mixed forest, mountain meadow, road, construction land, water area land and slope grade, which is slightly different from the actual value, indicating that the method has certain reliability.     

Paradigm and performance analysis of distributed frequent itemset mining algorithms based on Mapreduce

FrequentItemsetMining (FIM) is one of the most important data mining tasks and is the foundation of many data mining tasks. In Big Data era, centralized FIM algorithms cannot meet the needs of FIM for big data in terms of time and space, so Distributed Frequent Itemset Mining (DFIM) algorithms have been designed to meet the above challenges. In this paper, LocalGlobal and RedistributionMining which are two main paradigms of DFIM algorithm are discussed; Two algorithms of these paradigms on MapReduce named LG and RM are proposed while MapReduce is a popular distributed computing model, and also the related work is discussed. The experimental results show that the RM algorithm has better performance in terms of computation and scalability of sites, and can be used as the basis for designing the DFIM algorithm based on MapReduce. This paper also discusses the main ideas of improving the DFIM algorithms based on MapReduce.     

Government ecological governance management based on heterogeneous multi-processors and dynamic image sampling

With the advent of the governance economy era, traditional government governance models face enormous challenges. Governance management is the source of power to enter the era of the knowledge economy. This is a profound change that governments have needed to implement innovative government governance models, methods of social organization, and management. Governments in many countries are now adopting the construction of e-government as a strategy and response to demonstrate their priority development competitive advantage over the international competition and the challenges of the knowledge economy that spreads around the world. Therefore, the establishment of a new model of government governance has great theoretical and practical significance for impulse and government management innovation. The Dynamic Image Classification Algorithm (DICA) system is introduced in the implementation of the image processing technology and global disaster detection proposed here, and of this dynamic image classification algorithm (DICA) of the government, sector to extract more effectively. DICA is the most important task, or it is a strong shadow, proper access to the characteristics of the disaster area, and a false impact on the impact of the disaster, overcoming false alarms. It creates patches of post-disaster aerial image data from pre-tailoring and pre-adjustment training and satellite imagery. DICA training, all patches to government-managed data modified without delay or image data extraction. Two types of proposed systems are high-speed operation and accuracy (96.6%) and detection of earth disasters.     

(-)-6,6-乙二氧撑-1,10-二甲基-1(9)-八氢萘酮-2的合成

乙基乙烯基酮与4,4-乙二氧撑-2-甲基环乙酮2 c的手性亚胺进行不对称Michael加成,生成2,2-双取代环烷酮5,5经过克莱森缩合得到(-)-6,6-乙二氧撑-1,10-二甲基-1(9)-八氢萘酮-2(-)(1 c)。     

智能水滴算法研究

智能水滴算法是模拟自然界中河水与周围环境相互作用的过程而提出的一种智能计算方法。文章首先描述智能水滴算法的原理,然后综述智能水滴算法在多个研究领域的应用,最后对智能水滴算法进行总结与讨论。     

白芍中芍药总苷的提取工艺研究

用正交实验法对白芍中芍药总苷的提取工艺进行优选,考察乙醇体积分数、固液比、提取时间对白芍中芍药总苷提取率的影响。结果表明,在浸提3次条件下,得到最佳工艺条件为:乙醇浓度85%,固液比为1∶5(g/mL),提取时间3 h。在此条件下,白芍中芍药总苷提取率为1.366%。